P-497 High-concentration of E2 post-controlled ovarian hyperstimulation promotes cervical extracellular matrix degradation by up-regulating UBA52

Abstract Study question To explore the effect of high concentration E2 after controlled ovarian hyperstimulation (COH) on extracellular matrix cervical tissue in second trimester. Summary answer The COH promotes ECM degradation leading to insufficiency pregnancy ART by up-regulating UBA52. What is known already Assisted reproductive technology (ART) a double-edged sword. While effectively solving infertility, also associated with many obstetric complications like preterm birth, although, reason currently unclear. Cervical (CI) caused (ECM) one most important causes birth. All patients undergoing need receive (COH), sharp rise serum level E2, which has significant impact blood system, liver and kidney, but cervix design, size, duration 1.Retrospectively analyzed clinical data our center compare incidence between singleton natural pregnancy. 2. Establishing post-COH mice model, analyze how affect ECM. Participants/materials, setting, methods CI day human chorionic gonadotropin (HCG) pregnancies was compared. pregnant models were established detect investigate structure We detected overall expression collagen selected mark gene using RNA-seq. Relevant biological experiments used for verification 14/5000 Main results role chance significantly higher than that (7.80% vs. 3.54%, p<0.0001) so during those without (3333 ± 1116.61 pg/ml, n = 40 2437 1072.2 9, p=0.034). Furthermore, markedly (175.68 38.72 10 235.43 67.24 11, p=0.024) reduced significantly, DEGs related organization pathway enriched. Our indicated metalloproteinases (MMPs) should not be responsible cervix. Interestingly, ubiquitination type I increased compared control group both vitro vivo experiments. UBA52, component ubiquitin-encoding gene, been shown strongly upregulated among these genes (URGs), its demonstrated dependent. Also, an estrogen response element (ERE) UBA52 promoter region, direct binding ER ERE validated, implying might key regulator degradation. Limitations, reasons caution Firstly, findings still inevitably subject retrospective study limitations. Secondly, we found may target mouse COH, deeper mechanism Wider implications research provides evidence harmful function insufficiency, further underlines importance providing special care women who use Trial registration number applicable